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Bratislava Medical Journal Vol.120, No.1, p.86–88, 2019 |
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Title: An efficient induction protocol for deriving mature oligodendrocytes from human dental stem cells | ||
Author: Z. Bagheri-Hosseinabadi, H. Ghasemi Hamidabadi, M. Nazm Bojnordi | ||
Abstract: INTRODUCTION: This study examined the process of deriving mature oligodendrocytes from human dental pulp stem cells (hDPSCs) via addition of cerebrospinal fluid (CSF). METHODS: The hDPSCs were cultured in the presence of retinoic acid and CSF. The oligodendrocytes were confirmed using immunocytochemistry for specific glial markers, namely Olig2 and MBP markers. RESULTS: The differentiated oligodendrocytes were immunopositive for Olig2 and MBP markers at the end of induction phase. CONCLUSION: It is concluded that this study indicated the glial differentiation of hDPSCs in the presence of CSF and appropriate inducers, which is a usable therapeutic technique in neuroregenerative medicine (Fig. 3, Ref. 24). |
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Keywords: human dental pulp stem cells, oligodendrocyte, cerebrospinal fluid, retinoic acid | ||
Published online: 22-Jan-2019 | ||
Year: 2019, Volume: 120, Issue: 1 | Page From: 86, Page To: 88 | |
doi:10.4149/BLL_2019_013 |
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