Home 
Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.3, p.182–187,2020
Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.3, p.182–187,2020
Journal info
|
||||
Select Journal
Journals
Bratislava Medical Journal 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 Endocrine Regulations General Physiology and Biophysics Neoplasma Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Bratislava Medical Journal Vol.121, No.3, p.182–187,2020 |
||
| Title: Ribotypes in isolates and testing algorithm of C. difficile infections in the studied sample | ||
| Author: Z. Stofkova, E. Novakova, M. Novak | ||
| Abstract: BACKGROUND: Clostridium (Clostridioides) difficile is the most common pathogen of nosocomial and antibiotic-related diarrhea in health-care facilities. The aim of the analysis was to show the testing algorithm and to identify hypervirulent strains (suspected RT 027). METHODS: The retrospective analysis of patient samples suspected on CDI was carried out by a two-step algorithm. Biological specimens were analysed by GDH or culture, immunoenzymatic assay on toxins A/B and selected samples also by a real-time PCR. RESULTS: In 1006 specimen suspected on CDI, 202 specimens were evaluated as positive in the two-step algorithm. Conflicting results (64 C. difficile isolates) were tested in a three-step algorithm by a real-time PCR and revealed 59 toxigenic and non RT 027 ribotypes. Statistically significant dependence among the independent variables, such as: diagnostic parameters and length of hospitalization (p = 0.175) and C. difficile (suspected RT027) ribotypes was not found. CONCLUSION: The results of PCR ribotyping showed a high prevalence of hypervirulent and toxigenic ribotypes in the studied sample. A resistance to vancomycin was found in one isolate. The PCR method contributed to the rapid laboratory diagnosis and thus treatment of high risk patients or was used as a third step in in the case of unclear results of standard diagnostic methods (Tab. 1, Fig. 4, Ref. 18). Text in PDF www.elis.sk. |
||
| Keywords: toxigenic C. difficile, diagnostics, real-time PCR, ribotyping | ||
| Published online: 26-Feb-2020 | ||
| Year: 2020, Volume: 121, Issue: 3 | Page From: 182, Page To: 187 | |
| doi:10.4149/BLL_2020_027 |
||
|
|
 download file |
|