Home Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.3, p.182–187,2020

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

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Bratislava Medical Journal Vol.121, No.3, p.182–187,2020

Title: Ribotypes in isolates and testing algorithm of C. difficile infections in the studied sample
Author: Z. Stofkova, E. Novakova, M. Novak

Abstract: BACKGROUND: Clostridium (Clostridioides) difficile is the most common pathogen of nosocomial and antibiotic-related diarrhea in health-care facilities. The aim of the analysis was to show the testing algorithm and to identify hypervirulent strains (suspected RT 027).
METHODS: The retrospective analysis of patient samples suspected on CDI was carried out by a two-step algorithm. Biological specimens were analysed by GDH or culture, immunoenzymatic assay on toxins A/B and selected samples also by a real-time PCR.
RESULTS: In 1006 specimen suspected on CDI, 202 specimens were evaluated as positive in the two-step algorithm. Conflicting results (64 C. difficile isolates) were tested in a three-step algorithm by a real-time PCR and revealed 59 toxigenic and non RT 027 ribotypes. Statistically significant dependence among the independent variables, such as: diagnostic parameters and length of hospitalization (p = 0.175) and C. difficile (suspected RT027) ribotypes was not found.
CONCLUSION: The results of PCR ribotyping showed a high prevalence of hypervirulent and toxigenic ribotypes in the studied sample. A resistance to vancomycin was found in one isolate. The PCR method contributed to the rapid laboratory diagnosis and thus treatment of high risk patients or was used as a third step in in the case of unclear results of standard diagnostic methods
(Tab. 1, Fig. 4, Ref. 18). Text in PDF www.elis.sk.

Keywords: toxigenic C. difficile, diagnostics, real-time PCR, ribotyping
Published online: 26-Feb-2020
Year: 2020, Volume: 121, Issue: 3 Page From: 182, Page To: 187

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