Acta Virologica Vol.65, No.1, p.97-100, 2021

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Acta Virologica Vol.65, No.1, p.97-100, 2021
Title: Identification of Cucumber mosaic virus from Arisaema heterophyllum Blume in China
Author: Yanjing Zhang, Kaige Ma, Tingting Wu, Jingyu Liu, Jie Gao

Abstract: Arisaema heterophyllum Blume is a valuable medicinal plant in the Araceae family. The dried tuber of A. heterophyllum is used in the traditional Chinese medicine, Rhizoma Arisaematis, which is used to treat convulsions, inflammation and cancer. In 2017, typical mosaic virus-like symptoms were observed in A. heterophyllum in Jilin province, China. To further identify the pathogens, we conducted RT-PCR using virus- and genus-specific primers to amplify partial genome sequences of Cucumber mosaic virus (CMV), Tobamovirus and Potyvirus, respectively. The CMV primers showed specific amplification, but the Tobamovirus and Potyvirus primers did not. We further cloned and sequenced the 2b, MP and CP genes of the CMV-Ah isolate. Phylogenetic analysis showed the CMV-Ah isolate belonged to subgroup IB. To our knowledge, this is the first report of CMV infecting A. heterophyllum in China.

Keywords: Cucumber mosaic virus; Arisaema heterophyllum Blume; subgroup IB; phylogenetic analysis
	Published online: 23-Feb-2021
Year: 2021, Volume: 65, Issue: 1	Page From: 97, Page To: 100
doi:10.4149/av_2021_112
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