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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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neo_2021_201109N1196

Title: A novel antitumor peptide inhibits proliferation and migration and promotes apoptosis in glioma cells by regulating the MKK6/p38 signaling pathway
Author: Rong Wang, Bo-Wen Li, Nai-Yuan Shao, Dan-Ni Deng, Feng Zhi

Abstract: Protein- or peptide-based therapeutics have emerged as an innovative strategy for the treatment of cancer. Our previous research demonstrated that tripartite motif 9 short isoform (TRIM9s) is a tumor suppressor in glioma. In this report, we investigated whether a new peptide derived from TRIM9s, named T9sP, inhibits glioma progression and determined the possible molecular mechanism.
The CCK-8 proliferation assay was performed in LN229 and U251 glioma cells. The scratch-wound assay was used to determine the migration of the cells. Apoptosis was assessed by flow cytometry using Annexin V-FITC/PI double staining method. The relative protein expression levels were detected by immunoblot analysis.
The cell-penetrating peptide TAT was fused with T9sP to form TAT-T9sP. TAT-T9sP efficiently penetrated through the cell membrane of both LN229 and U251 cells. TAT-T9sP inhibited proliferation and migration and promoted apoptosis of glioma cells. TAT-T9sP activated p38 signaling by upregulating MKK6, and a p38 inhibitor, SB203580, reversed the inhibitory effects of TAT-T9sP on glioma cells.
These results indicated the potential of TAT-T9sP for the development of a new anti-glioma medicine.

Keywords: T9sP; glioma; p38; MKK6
Published online: 13-Apr-2021
Year: , Volume: , Issue: Page From: , Page To:
doi:10.4149/neo_2021_201109N1196


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