Home Bratislava Medical Journal 2024 Bratislava Medical Journal Vol.125, No.4, p.219–222, 2024

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.564

 

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Bratislava Medical Journal Vol.125, No.4, p.219–222, 2024

Title: Efficacy of sample collection without virus transport medium in suspected enteroviral infections for molecular diagnosis
Author: Maria BORSANYIOVA, Shubhada BOPEGAMAGE, Sandor George VARI
Abstract: Clinical swabs with suspected viral infection are usually transported in virus transport medium (VMT). During epidemics/pandemics, tampons without VTM would be more suitable for saving space and cost. This study was conducted to verify the applicability of throat swabs without VTM in the diagnosis/screening of enteroviral infections by polymerase chain reaction (PCR) in a volunteer study group. Three different swab types were used in 40 volunteers: swabs with two different tips (cotton- or synthetic-tipped) without VTM and standard synthetic tips with VTM. The swabs were processed immediately or after 12 days of storage at either –80°C or +4°C. The molecular analysis included viral RNA extraction, and combination of reverse transcriptase PCR and nested PCR. Enteroviral RNA was detected in 15% (6/40) of the studied volunteers. When processed immediately, the results for all three swab types were compatible. Swabs without VTM may be used for collection of clinical samples in the diagnosis of suspected enteroviral infections or as potential screening tools for enteroviruses (Tab. 2, Ref. 15).
Keywords: enterovirus infection, swab, transport medium, PCR, molecular diagnostics
Published online: 26-Jan-2024
Year: 2024, Volume: 125, Issue: 4 Page From: 219, Page To: 222
doi:10.4149/BLL_2024_33


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